Biofunctionalization of two-dimensional nanomaterials for the needs of regenerative biomedicine

Short description

The primary objective of this project is to establish and experimentally validate the principles for biofunctionalizing two-dimensional nanomaterials (MXenes) and to elucidate the mechanisms underlying their interactions with biological systems. This will facilitate the development of novel biocompatible and bioactive materials for applications in regenerative biomedicine.

The project involves investigating the dynamics and kinetics of the interactions between transition metal carbide- and nitride-based MXenes and serum proteins, with a specific focus on the formation, composition, and stability of the protein corona.

A methodology for MXene biofunctionalization will be developed via the surface immobilization of model proteins and biologically active growth factors (specifically FGF2 and FGF2-STAB).

We will examine the impact of MXene surface modifications on their intracellular uptake and assess the in vitro biological effects of these biofunctionalized MXenes on both normal and malignant cell lines.

Furthermore, the project encompasses a comprehensive suite of in vivo experiments designed to evaluate the biological activity and potential regenerative efficacy of the biofunctionalized MXenes.

The anticipated outcomes will establish a robust scientific foundation for the development of next-generation biocompatible nanomaterials for medical applications, including targeted drug delivery systems, tissue engineering scaffolds, and advanced biosensors.