Biomedical research center
Cell Culture:
Cell culture laboratory equipped with modern facilities for wide range of research with primary and certified cell lines.
about the laboratory
Laboratory provide following research activities
- Cytotoxicity of new drugs and substances
- Assessment of biocompatibility of new biomaterials
- Evaluation of anticancer activities
- Tissue engineering experiments
Following assay are used in research
- Resazurin reduction assay
- MTT assay
- Collagen deposition assay
- ALP assay
- Ca-deposition say
Laboratory equipped with
- Laminar box Porsa Ukraine
- Fluorescent microscope Axio Lab. A1
- Plate-reader Multiscan FC
- Visualization system EVOS ХL Core Imagine System
- CO2 boxes, refrigerators, -85 0С freezer
Cell culture laboratory owns following cell lines
- HOS – Homo sapience bone osteosarcoma (https://www.lgcstandards-atcc.org/products/all/CRL-1543.aspx?geo_country=ua);
- U2OS – Homo sapience bone osteosarcoma (https://www.lgcstandards-atcc.org/products/all/HTB-96.aspx?geo_country=ua)
- CHO-WT – Chinese Hamster Ovary Cell (https://www.lgcstandards-atcc.org/products/all/CCL-61.aspx?geo_country=ua)
- CHO-huCEACAM1-4L – Chinese Hamster Ovary Cell with human CEACAM-1 expression (https://patents.google.com/patent/EP3272354A1/en)
- MKN28 – Well differentiated human gastric adenocarcinoma cell line (https://www.ebi.ac.uk/ols/ontologies/efo/terms?short_form=EFO_0002831)
- MKN28-huCEACAM1-4L – human CEACAM-1 expression (https://patents.google.com/patent/EP3272354A1/en)
Additionally, we have following primary cell lines selected from human subjects:
- osteoblasts (selected from periosteum)
- fibroblasts (selected from skin)
- colorectal cancer (selected from patient with colorectal cancer)
For special research purpose, we can select different cells from both animals and patients.