Short description

The project aims to develop an innovative approach to optimize wound healing by creating a biopolymeric nanofibrous multilayer coating with a multifunctional orientation. The approach involves interdisciplinary cooperation, including materials science, nanotechnology, organic chemistry, and medical-biological sciences.

The project will create a bioactive polymeric nanofibrous membrane with silver nanoparticles that enhances antibacterial action and delivers growth factors for effective fibroblast migration. The study will evaluate the cytotoxicity of biopolymer matrices of different compositions and their effectiveness based on the concentration of added nanoparticles.

New bioactive multilayer coatings will be assessed for their effectiveness at all stages of wound healing in the multifunctional approach to optimizing wound healing. This is achieved by stepwise influence on the process through layer-by-layer release of components to ensure the optimization of all components of the healing process, unlike unidirectional hemostatic agents.

The goal of the project is to determine the possibility of using new three-layer nanofibrous membranes (produced by electrospinning) to provide hemostasis and antibacterial functions in the lower layer (chitosan-AgNPs), retain moisture and absorb exudate in the middle layer (chitosan-PVA), and promote tissue formation in the upper layer (PCL/PVA-FGF2-STAB).

The comprehensive application of these approaches will determine the rational ratio of polymers in the layers of the nanofibrous membrane and the optimal amounts of growth factors and nanoparticles for the development of bioactive polymeric constructs with tissue engineering hemostatic properties.