Biomedical research center

RESEARCH PROJECTS:

Projects in progress:

Funding Organization: EU, Horizon 2020, MSCA-RISE-2017

Overall Budget: 1 080 000 EUR

Project Duration: 2018-2021

SSU budget: 214 000 EUR 

Partner organizations:

Research Team:

1. University of Latvia (Latvia)

1.   Associate      Prof.,      Dr.     Viktoriia

Holubnicha, PhD

2. Sheffield University (UK)

2. Dr. Viktoriia Korniienko, PhD

3. Reggio-Modena University (Italy)

3. Dr. Aleksei Kalinkevich, PhD

4. Osteoplant R&D (Poland)

4. Dr. Aleksandr Solodovnyk, PhD

5. NanoPharma (Czech Republic)

5. Dr. Aleksandr Oleshko, PhD

6. Matecc (Estonia)

6. Dr. Volodymyr Deineka, PhD student

 

7. Dr. Oksana Kalinkevich

 

8. Dr. Evhenia Husak

 

9. Irina Liubchak, student

 Principal Investigator from SSU: Prof. Maksym Pogorielov

Short description. The project NanoSurf is targeted to strengthen international and intersectoral collaboration in dental implant research; sharing new ideas and knowledge transfer from research to market and vice versa. We will investigate nanostructured metal oxide coatings, 3D scaffolds, obtained by electrospinning deposition of organic nanofibers and lazer patterning of the implant surface using biotechnology, cell engineering and nanotechnology. Interdisciplinary project research and innovation goals are targeted to develop a new class of dental implants with advanced mechanical properties and improved surfaces, treated with nanotechnological methods which will demonstrate high biocompatibility, antibacterial properties and integration with a patient’s bone. The developed devices will simplify dental surgery and avoid bacterial inflammatory complications after the implant surgery. This can provide better dental services and improve health of EU society. The project partners will provide research and training activities in the fields of fabrication and characterization of Zirconium-Titanium (ZrTi) alloy-based dental implants, sol-gel deposition of metal oxides, biopolymers and organic/inorganic nanolaminates, laser patterning, electrospinning, structural characterization, cell engineering, modeling analysis and commercialization of scientific achievements from research results to final product.

Research and management training will be provided to experienced and early stage researchers to strengthen their personal skills, improve their track record and expertise via new scientific papers and conference presentations and strengthen a development of EU research human resources. Long lasting collaboration between partners, based on co-supervising students and preparation of novel collaborative project proposals is foreseen. Dissemination of the research and innovation project results will make an impact on development of EU research potential in the fields of bio-, nanotechnology and applied science.

Funding Organization: Ministry of Education and Science of Ukraine

Budget: 2 200 000 UAH

Project Duration: 2019-2021

Principal Investigator: Dr. Oleksandr Oleshko, PhD

Research Team:

  1. Volodymyr Deineka, PhD student
  2. Evhenia Husak
  3. Bohdan Drygval, PhD Student
  4. Viktoriia Korniienko, PhD

Short description.

Biodegradable alloys of Mg, Zn and Fe in last decades have become valuable alternative for traditional metals in orthopedics, surgery and cardiovascular surgery due to the opportunity of full elimination after completing their function. Unfortunately, the main problem for degradable metal`s clinical application remains its uncontrolled resorption, especially on the first stages after the implantation, what causes a number of clinical complications. Metallurgic methods (new alloys` creation and post-cast treatment), formation of protective coatings, particularly calcium phosphate ceramics, are used for solving this problem. This research is focused on creation of coating on the surface of Mg-based implants using the method of plasma electrooxidation. In this study, different model solutions with an addition of nanoparticles will be used for biocompatibility enhancement and optimal process modes (time, voltage and power of current) will be established. In addition, degradation time and elements` release dynamic will be studied depending on the type and mode of coatings. Biological response will be evaluated using the commercial available cellular lines MG63 (cells of human osteosarcoma) and MLO-A5 (osteoblastic bone cells).

Funding Organization: Ministry of Education and Science of Ukraine

Budget: 1 200 000 UAH

Project Duration: 2019-2021

Principal Investigator: Prof. Maksym Pogorielov

Research Team:

  1. Viktoriia Korniienko, PhD
  2. Oleksandr Oleshko, PhD
  3. Volodymyr Deineka, PhD student
  4. Evhenia Husak
  5. Associate Prof., Dr. Viktoriia Holubnicha, PhD
  6. Irina Liubchak, student

Short description.

The purpose of the project is to determine the features of the formation of the functional surface of metal implants using the sol-gel method and Plasma Electrolytic Oxidation and to assess mechanisms for surface interaction with osteogenic cells.

During the project implementation, we will improve the technique of Plasma Electrolytic Oxidation of metal implant surfaces using principally new solutions, in particular Ca(HCOO)2, Mg(CH3COO)2, H3PO4 with the addition of hydroxyapatite and nanoparticles of metal oxides. For the first time, a sol-gel method will be used for titanium-zirconium alloys to form an active layer on the surface that have undergone a plasma electoxidation process that will allow the formation of new chemical groups that may have an effect on the adhesion of proteins and osteogenic cells.

The osteogenic potential of coated implants surfaces will be tested on commercially available cell lines MG63 (human osteosarcoma cells) and MLO-A5 (osteoblastic, bone cells). The response of the cells will be evaluated by the presence of matrix mineralization, collagen production, calcium and alkaline phosphatase.

Funding Organization: Ministry of Education and Science of Ukraine

Budget: 1 578,327 UAH

Project Duration: 2020-2022

Principal Investigator: Dr. Sergiy Kyrylenko, PhD

Research Team:

  1. Dr. Roman Viter, PhD
  2. Dr.Viktoriia Korniienko, PhD
  3. Dr. Aleksandr Solodovnyk, PhD
  4. Dr. Oksana Pogorielova, PhD
  5. Dr. Viktoriia Fedorenko, PhD

Short description

Peripheral nerve injuries cause painful neuropathies and neuromas, poor sensation, and weakness and paralysis for patients due to the loss of function in damaged motor and sensory axons. Over the past decades, regeneration of damaged peripheral nerves using nerve tissue grafts has been extensively studied and significant progress has been made. Shortcomings of the use of grafts inspired the development of alternative, artificial nerve guidance conduits. Nerve Guide Conduits (NGC) are natural or artificial channels guiding axonal regrowth to facilitate nerve regeneration. Use of nerve conduits is preferable over suture repair and nerve grafting, as the functional recovery rates are above 80%. However, current commercial nerve conduits repair nerve defects of less than 2 cm. Nerve regeneration tends to fail over longer distances.

Therefore, actual task of the project is forming novel effective long structures (3-5 cm) NGCs with high active surface area, biocompatibility, biodegradability, permeability, combined with high electrical conductivity and characteristic optical absorption.

The project sets up the following scientific objectives:

  1. To develop functional 2D matrix (2D mat) with advanced electrical, optical and biocompatible properties, based on electrospun nanofibers and conductive materials (Ag, Au nanoparticles, MXenes and conductive polymers);
  2. To investigate the influence of stimulation of Schwann cells (SCs) and Neural crest derived stem cells (NC-MSCs) cell growth in 2D matrix by electrical field and light from VIS-NIR region;
  3. To investigate biocompatibility, effect for cell growth and guided proliferation using relevant cell types (Schwann cells, Neural crest derived stem cells) in NGCs.
  4. To provide in vivo tests of NGCs on large laboratory animals for clinical effectiveness, including electrical stimulations in different modes.

 

Funding Organization: Ministry of Education and Science of Ukraine

Budget: 1 200 000 UAH

Project Duration: 2019-2021

Principal Investigator: Prof. Maksym Pogorielov

Research Team:

  1. Viktoriia Korniienko, PhD
  2. Oleksandr Oleshko, PhD
  3. Volodymyr Deineka, PhD student
  4. Evhenia Husak
  5. Associate Prof., Dr. Viktoriia Holubnicha, PhD
  6. Irina Liubchak, student

Short description.

The purpose of the project is to determine the features of the formation of the functional surface of metal implants using the sol-gel method and Plasma Electrolytic Oxidation and to assess mechanisms for surface interaction with osteogenic cells.

During the project implementation, we will improve the technique of Plasma Electrolytic Oxidation of metal implant surfaces using principally new solutions, in particular Ca(HCOO)2, Mg(CH3COO)2, H3PO4 with the addition of hydroxyapatite and nanoparticles of metal oxides. For the first time, a sol-gel method will be used for titanium-zirconium alloys to form an active layer on the surface that have undergone a plasma electoxidation process that will allow the formation of new chemical groups that may have an effect on the adhesion of proteins and osteogenic cells.

The osteogenic potential of coated implants surfaces will be tested on commercially available cell lines MG63 (human osteosarcoma cells) and MLO-A5 (osteoblastic, bone cells). The response of the cells will be evaluated by the presence of matrix mineralization, collagen production, calcium and alkaline phosphatase.

All the rest listed in the page

Funding Organization: Ministry of Education and Science of Ukraine

Budget: 1 450 000 UAH

Project Duration: 2018-2020

Principal Investigator: Prof. Andrii Loboda

Research Team:

  1. Associate Prof., Dr. Viktoriia Holubnicha, PhD
  2. Viktoriia Korniienko, PhD
  3. Aleksei Kalinkevich, PhD
  4. Oksana Kalinkevich
  5. Evhenia Husak

Short description.

According to the European Center for Disease Control and Prevention (ECDC), around 25,000 people in Europe die from infections caused by resistant microorganisms, and according to the British government data, mortality for this reason in the world is up to 500,000 per year. The study of mechanisms of action and effectiveness of new classes of compounds that have antibacterial action is an actual problem of modern science.

The purpose of the project is to assess the mechanism of action and efficiency of nanocomposites “chitosan-nanometal” against the most common antibiotic-resistant clinical isolates.

During the project, the study of antibiotic resistant strains isolated from health facilities as well as identification of the most common clinical isolates list will be conducted . In this work, the effectiveness and mechanism of action of synthesized nanoparticles (copper, silver and zinc) action, and created complexes of “chitosan-nanometal” will be determined. The features of genotoxicity and cytotoxicity of the most effective complexes will be determined, and the possibility of reducing the number of side effects when using chitosan will be examined.

Funding Organization: Ministry of Education and Science of Ukraine

Budget: 800 000 UAH

Project Duration: 2016-2018

Principal Investigator: Prof. Maksym Pogorielov

Research Team:

  1. Viktoriia Korniienko, PhD
  2. Oleksandr Oleshko, PhD
  3. Volodymyr Deineka, PhD student
  4. Evhenia Husak
  5. Irina Liubchak, student

Short description.

The aim of current project is to create medical supplies for effective haemostasis during the military action, man-made disasters, industrial and road accidents as well as hemostatic drugs to stop bleeding from parenchymal organs in surgery. Acute massive blood loss is the main cause of death in the prehospital phase both in civil case and battlefield, bleeding from parenchymal organs is a major cause of mortality during trauma and abdominal injuries. Today in Ukraine registered local hemostatic agents based on kaolin and iron salts, and sporadic drugs to stop the bleeding from parenchymal organs – “hemostatic sponge” and “Taсhokomb.” Implementation of this project provides the creation of highly effective medical supplies to stop bleeding from the peripheral blood vessels, including femoral, tibial and brachial artery and surgical sponges to control blood loss after injury of parenchymal organs. New tools provide the ability to stop massive bleeding in case of injury of different genesis – gunshot wounds, sharp and blunt mechanical injuries and achieve effective hemostasis during surgery. Thus, the cost of production facilities will be much lower than foreign analogues.

Funding Organization: Silesian University of Technology, Subcontracting ZP/008857/18

Budget: 33 500 EUR

Project Duration: 2018-2019

Principal Investigator: Prof. Maksym Pogorielov

Research Team:

  1. Associate Prof., Dr. Viktoriia Holubnicha, PhD
  2. Aleksandr Oleshko, PhD
  3. Viktoriia Korniienko, PhD
  4. Volodymyr Deineka, PhD student
  5. Evhenia Husak
  6. Julia Dudko, student

Short description.

This project is aimed to assess cell attachment and proliferation rate of osteoblasts and MSCs on modified surface of Ti-based implants.

Funding Organization: Sumy State University

Budget: 15 000 UAH

Project Duration: 2019-2020

Principal Investigator: Dr. Volodymyr Deineka, PhD Student

Research Team:

  1. Yaroslav Znamenshchykov
  2. Ivan Lytvynenko PhD student
  3. Oksana Kalinkevich

Short description.

The project will be implemented in cooperation with the Department of Electronics and Computer Engineering and the Department of Public Health of SSU. A 3D printer modification will be designed for printing of gel biopolymers. A method will be developed for the formation of three- dimensional biopolymer scaffolds and experimentally selected type of polymer and its characteristics are determined for optimum printing speed polymerization. An optimal three- dimensional structure of the scaffold will be created for the possible population of its cell culture and the characterization of the surface and internal structure of the sample by SEM. The final phase of the project involves cytotoxicity determination of the scaffolds and the possibility of adhesion of the mesenchymal stem and somatic cells on its surface.

Funding Organization: Sumy State University

Budget: 14 900 UAH

Project Duration: 2019

Principal Investigator: Petro Myronov, Ph.D. student

Research Team:

  1. Aziza Yusupova, student
  2. Anton Savchenko, student
  3. Evhenia Husak
  4. Irina Dudko

Short description

The aim of the current project is to Investigate the chemical composition and ultrastructure of the cell wall of antibiotic-resistant clinical isolates E.Coli under the influence of the chitosan-nanometal composites. The study of nanomaterial’s antibacterial activity is a promising direction in combating resistance to antibiotics. It is possible to reduce the effective concentration of metal in combination with chitosan, which has its own antibacterial activity. It is planned to investigate polyresistant isolates before and after using of biocomposites, to improve methods of isolating the bacterial cell wall structures and their chemical characteristics on the example of E.coli lipopolysaccharides. Combining chitosan with different metal concentrations will reveal minimal inhibitory concentrations of composites relative to resistant clinical isolates. The obtained results will allow to deepen the existing knowledge about mechanisms of antibacterial action of the complex “chitosan- nanometal” and will serve as the basis for further development of antiseptic agents.

Funding Organization: Sumy State University

Budget: 15 000 UAH

Project Duration: 2019

Principal Investigators: Irina Liubchak, student

Research Team:

  1. Olha Vorozhko, student
  2. Julia Dudko, student

Short description.

The aim of current project is to evaluate the properties of 3D chitosan materials, manufactured according to the principles of “Green Chemistry” with the help of microwave irradiation. Chitosan is a non-toxic organic polymer and has most of the qualities (biocompatibility, an ability to completely degrade under the physiological conditions, high regenerative potential, antimicrobial properties etc.) which make it a perspective material for tissue regeneration purposes. However, the creation of versatile scaffold for tissue engineering often requires multistage chemical reactions with the use of toxic substances. Therefore, physical methods possess a good alternative for 3D scaffolds synthesis. The impact of new manufacturing method would be investigated based on results of cytotoxicity, microbiological and blood clotting experiments.

  1. https://www.wcrf.org
  2. http://gicr.iarc.fr/en/
  3. https://www.who.int
  4. Dankner, M, Gray-Owen, S. D., Huang, Y-H., Blumberg, R. S., Beauchemin N. CEACAM1 as a multi-purpose target for cancer Oncoimmunology. 2017; 6(7): e1328336. doi: 10.1080/2162402X.2017.1328336. PMCID: PMC5543821. PMID: 28811966
  5. Helfrich I. & Singer BB. Size Matters: The Functional Role of the CEACAM1 Isoform Signature and Its Impact for NK Cell-Mediated Killing in Melanoma. Cancers (Basel). 2019 Mar 13;11(3). pii: E356. doi: 3390/cancers11030356. Review. PMID: 30871206
  6. Kotagiri, N.; Sudlow, G. P.; Akers, W. J.; Achilefu, S. Breaking the Depth Dependency of Phototherapy with Cerenkov Radiation and Low-Radiance-Responsive Nanophotosensitizers. Nat. Nanotechnol. 2015, 10, 370-379.
  7. Yang, K.; Feng, ; Liu, Z. Stimuli Responsive Drug Delivery Systems Based On NanoGraphene for Cancer Therapy. Adv. Drug Deliver. Rev. 2016, 105, 228-241.
  8. Fusco et Theranostics 2020 doi:10.7150/thno.4006
  9. Naguib, M., Kurtoglu, M., Presser, V., Lu, J., Niu, J., Heon, , et al. (2011). Twodimensional nanocrystals produced by exfoliation of Ti3AlC2. Adv. Mater. 23, 4248–4253. doi: 10.1002/chin.201152200
  10. Wang, S.; Li, X.; Chen, ; Cai, X.; Yao, H.; Gao, W.; Zheng, Y.; An, X.; Shi, J.; Chen, H. A Facile One-Pot Synthesis of a Two-Dimensional MoS2/Bi2S3 Composite Theranostic Nanosystem for Multi-Modality Tumor Imaging and Therapy. Adv. Mater. 2015, 27, 2775-2782.
  11. Tao, W.; Zhu, X.; Yu, X.; Zeng, ; Xiao, Q.; Zhang, X.; Ji, X.; Wang, X.; Shi, J.; Zhang, H.; Mei, L. Black Phosphorus Nanosheets as a Robust Delivery Platform for Cancer Theranostics. Adv. Mater. 2017, 29, 1603276.
  12. Cai, Y.; Liang, ; Tang, Q.; Yang, X.; Si, W.; Huang, W.; Zhang, Q.; Dong, X. Diketopyrrolopyrrole−Triphenylamine Organic Nanoparticles as Multifunctional Reagents for Photoacoustic Imaging Guided Photodynamic/Photothermal Synergistic Tumor Therapy. ACS Nano 2017, 11, 1054−1063
  13. Agostinis, P., Berg, , Cengel, K. A., Foster, T. H., Girotti, A. W., Gollnick, S. O., et al. (2011). Photodynamic therapy of cancer: an update. CA Cancer J. Clin. 61, 250–281. doi: 10.3322/caac.20114
  14. Gazzi A, Fusco L, Khan A, Bedognetti D, Zavan B, Vitale F, Yilmazer A and Delogu LG (2019) Photodynamic Therapy Based on Graphene and MXene in Cancer Theranostics. Front. Bioeng. Biotechnol. 7:295. doi: 10.3389/fbioe.2019.00295
  15. Han Lin, Yu Chen, Jianlin Shi. Insights into 2D MXenes for Versatile Biomedical Applications: Current Advances and Challenges Ahead. Advanced Science. Volume5, Issue10, October 2018, 1800518
  16. Anasori, M. R. Lukatskaya, Y. Gogotsi, Nat. Rev. Mater. 2017, 2, 16098
  17. Chaudhuri, K., Alhabeb, M., Wang, , Shalaev, V. M., Gogotsi, Y., & Boltasseva, A. (2018). Highly Broadband Absorber Using Plasmonic Titanium Carbide (MXene). ACS Photonics, 5(3), 1115–1122. doi:10.1021/acsphotonics.7b01439
  18. Wu L, Lu X, Wu Z-S, Dong Y, Wang X, Zheng S and Chen J (2018) 2D transition metal carbide MXene as a robust biosensing platform for enzyme immobilization and ultrasensitive detection of phenol. Biosensors and Bioelectronincs, 107:69–75. PMID: 29448223 DOI: 1016/j.bios.2018.02.021
  19. Kumar S, Lei Y, Alshareef NH, Quevedo-Lopez MA and Salama (2018). Biofunctionalized Two-Dimensional Ti 3 C 2 MXenes for Ultrasensitive Detection of Cancer Biomarker. Biosensors and Bioelectronincs, 121:243-249. PMID: 30219724 DOI: 10.1016/j.bios.2018.08.076
  1. https://www.wcrf.org
  2. http://gicr.iarc.fr/en/
  3. https://www.who.int
  4. Dankner, M, Gray-Owen, S. D., Huang, Y-H., Blumberg, R. S., Beauchemin N. CEACAM1 as a multi-purpose target for cancer Oncoimmunology. 2017; 6(7): e1328336. doi: 10.1080/2162402X.2017.1328336. PMCID: PMC5543821. PMID: 28811966
  5. Helfrich I. & Singer BB. Size Matters: The Functional Role of the CEACAM1 Isoform Signature and Its Impact for NK Cell-Mediated Killing in Melanoma. Cancers (Basel). 2019 Mar 13;11(3). pii: E356. doi: 3390/cancers11030356. Review. PMID: 30871206
  6. Kotagiri, N.; Sudlow, G. P.; Akers, W. J.; Achilefu, S. Breaking the Depth Dependency of Phototherapy with Cerenkov Radiation and Low-Radiance-Responsive Nanophotosensitizers. Nat. Nanotechnol. 2015, 10, 370-379.
  7. Yang, K.; Feng, ; Liu, Z. Stimuli Responsive Drug Delivery Systems Based On NanoGraphene for Cancer Therapy. Adv. Drug Deliver. Rev. 2016, 105, 228-241.
  8. Fusco et Theranostics 2020 doi:10.7150/thno.4006
  9. Naguib, M., Kurtoglu, M., Presser, V., Lu, J., Niu, J., Heon, , et al. (2011). Twodimensional nanocrystals produced by exfoliation of Ti3AlC2. Adv. Mater. 23, 4248–4253. doi: 10.1002/chin.201152200
  10. Wang, S.; Li, X.; Chen, ; Cai, X.; Yao, H.; Gao, W.; Zheng, Y.; An, X.; Shi, J.; Chen, H. A Facile One-Pot Synthesis of a Two-Dimensional MoS2/Bi2S3 Composite Theranostic Nanosystem for Multi-Modality Tumor Imaging and Therapy. Adv. Mater. 2015, 27, 2775-2782.
  11. Tao, W.; Zhu, X.; Yu, X.; Zeng, ; Xiao, Q.; Zhang, X.; Ji, X.; Wang, X.; Shi, J.; Zhang, H.; Mei, L. Black Phosphorus Nanosheets as a Robust Delivery Platform for Cancer Theranostics. Adv. Mater. 2017, 29, 1603276.
  12. Cai, Y.; Liang, ; Tang, Q.; Yang, X.; Si, W.; Huang, W.; Zhang, Q.; Dong, X. Diketopyrrolopyrrole−Triphenylamine Organic Nanoparticles as Multifunctional Reagents for Photoacoustic Imaging Guided Photodynamic/Photothermal Synergistic Tumor Therapy. ACS Nano 2017, 11, 1054−1063
  13. Agostinis, P., Berg, , Cengel, K. A., Foster, T. H., Girotti, A. W., Gollnick, S. O., et al. (2011). Photodynamic therapy of cancer: an update. CA Cancer J. Clin. 61, 250–281. doi: 10.3322/caac.20114
  14. Gazzi A, Fusco L, Khan A, Bedognetti D, Zavan B, Vitale F, Yilmazer A and Delogu LG (2019) Photodynamic Therapy Based on Graphene and MXene in Cancer Theranostics. Front. Bioeng. Biotechnol. 7:295. doi: 10.3389/fbioe.2019.00295
  15. Han Lin, Yu Chen, Jianlin Shi. Insights into 2D MXenes for Versatile Biomedical Applications: Current Advances and Challenges Ahead. Advanced Science. Volume5, Issue10, October 2018, 1800518
  16. Anasori, M. R. Lukatskaya, Y. Gogotsi, Nat. Rev. Mater. 2017, 2, 16098
  17. Chaudhuri, K., Alhabeb, M., Wang, , Shalaev, V. M., Gogotsi, Y., & Boltasseva, A. (2018). Highly Broadband Absorber Using Plasmonic Titanium Carbide (MXene). ACS Photonics, 5(3), 1115–1122. doi:10.1021/acsphotonics.7b01439
  18. Wu L, Lu X, Wu Z-S, Dong Y, Wang X, Zheng S and Chen J (2018) 2D transition metal carbide MXene as a robust biosensing platform for enzyme immobilization and ultrasensitive detection of phenol. Biosensors and Bioelectronincs, 107:69–75. PMID: 29448223 DOI: 1016/j.bios.2018.02.021
  19. Kumar S, Lei Y, Alshareef NH, Quevedo-Lopez MA and Salama (2018). Biofunctionalized Two-Dimensional Ti 3 C 2 MXenes for Ultrasensitive Detection of Cancer Biomarker. Biosensors and Bioelectronincs, 121:243-249. PMID: 30219724 DOI: 10.1016/j.bios.2018.08.076

References – Project 3D-Sub NRFU2021

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